Use of a baculovirus to express and exogenous gene in a mammalian cell
AssigneeThe General Hospital Corporation
InventorFrederick M. Boyce
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Abstract
Disclosed is a method of expressing an exogenous gene in a mammalian cell, involving infecting the cell with a baculovirus whose genome carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed is a method of treating a gene deficiency disorder in a mammal by providing to a cell a therapeutically effective amount of a baculovirus whose genome carries an exogenous gene and growing the cell under conditions such that the exogenous gene is expressed in the mammal.
Claims36
A method of expressing an exogenous gene in a mammalian cell in vitro, said method comprising:
- a) introducing into the cell a baculovirus whose genome comprises said exogenous gene;
- and
- b) allowing said cell to live under conditions such that said exogenous gene is expressed.
A nucleic acid comprising:
- a baculovirus genome;
- an exogenous mammalian gene;
- and
- an exogenous promoter selected from the group consisting of a mammalian promoter and a Hepatitis B viral promoter, wherein said gene is operably linked to said promoter.
A cell, in vitro that contains a nucleic acid, wherein said nucleic acid comprises:
- a baculovirus genome;
- an exogenous mammalian gene;
- and
- an exogenous promoter selected from the group consisting of a mammalian promoter and a Hepatitis B viral promoter, wherein said gene is operably linked to said promoter.
Dependencies are inferred from the claim text.