Surface expression libraries of heteromeric receptors

A composition of matter comprising a plurality of cells containing only operatively combined vectors having diverse combinations of first and second DNA sequences encoding functional portions of variable heavy and variable light chains of an antibody, one or both of said functional portions of said variable heavy and variable light chains being expressed as fusion proteins on the surface of a cell or a bacteriophage, said operatively combined vectors being formed by the recombination of first and second vectors having two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second DNA sequences, said recombination effected between the two pairs of restriction sites.

A cloning system for the coexpression of two or more DNA sequences encoding polypeptides which form a heteromeric receptor of the immunoglobulin superfamily, comprising a set of first vectors having a diverse population of first DNA sequences and a set of second vectors having a diverse population of second DNA sequences, said diverse populations of first and second DNA sequences encoding polypeptides which form heteromeric receptors of the immunoglobulin superfamily and said first and second vectors having two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second populations of DNA sequences so as to allow only the operational combination of vector sequences containing said first and second DNA sequences.

A cloning system for the coexpression of two or more DNA sequences encoding functional portions of variable heavy and variable light chains which form an antibody, comprising a set of first vectors having a diverse population of first DNA sequences and a set of second vectors having a diverse population of second DNA sequences, said first and second vectors having two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second populations of DNA sequences so as to allow only the operational combination of vector sequences containing said first and second DNA sequences.

A plurality of expression vectors containing a plurality of first and second DNA sequences encoding a plurality of first and second polypeptides, said expression vectors formed from two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second DNA sequences, wherein any random combination of a first DNA sequence and a second DNA sequence can only be operatively linked to encode first and second polypeptides that form a plurality of heteromeric receptors of the immunoglobulin superfamily, said plurality of heteromeric receptors of the immunoglobulin superfamily being characterized by at least one heteromeric receptor exhibiting binding activity toward a preselected molecule, said DNA sequences being further operatively linked to at least one gene encoding a surface protein of a cell or a bacteriophage.

A plurality of expression vectors containing a plurality of first and second DNA sequences encoding a plurality of functional portions of variable heavy and variable light chains of an antibody, said expression vectors formed from two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second DNA sequences, wherein any random combination of a first DNA sequence and a second DNA sequence can only be operatively linked to encode functional portions of the variable heavy and variable light chains of an antibody that form a plurality of antibodies, said plurality of antibodies being characterized by at least one antibody exhibiting binding activity toward a preselected molecule, said DNA sequences being further operatively linked to at least one gene encoding a surface protein of a cell or a bacteriophage.

A composition of matter comprising a plurality of cells containing only operatively combined vectors having diverse combinations of first and second DNA sequences encoding functional binding portions of the first chain and second chain of a T cell receptor, one or both of said functional binding portions of said first chain and second chain being expressed as fusion proteins on the surface of a cell or a bacteriophage, said operatively combined vectors being formed by the recombination of first and second vectors having two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second DNA sequences, said recombination effected between the two pairs of restriction sites.

A cloning system for the coexpression of two or more DNA sequences encoding functional binding portions of the first chain and second chain which form a T cell receptor, comprising a set of first vectors having a diverse population of first DNA sequences and a set of second vectors having a diverse population of second DNA sequences, said first and second vectors having two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second populations of DNA sequences so as to allow only the operational combination of vector sequences containing said first and second DNA sequences.

A plurality of expression vectors containing a plurality of first and second DNA sequences encoding a plurality of functional binding portions of the first chain and second chain of a T cell receptor, said expression vectors formed from two pairs of restriction sites symmetrically oriented about a cloning site for containing said first and second DNA sequences, wherein any random combination of a first DNA sequence and a second DNA sequence can only be operatively linked to encode functional binding portions of the first chain and second chain of a T cell receptor that form a plurality of T cell receptors, said plurality of T cell receptors being characterized by at least one T cell receptor exhibiting binding activity toward a preselected molecule, said DNA sequences being further operatively linked to at least one gene encoding a surface protein of a cell or a bacteriophage.