Back to results
US 5,869,283 AGranted
Expression cassette operable in a recombinant host
AssigneeRoussel Uclaf
InventorsHerman Slijkhuis, Eric Bastiaan Smaal, Gerardus Cornelis Maria Selten
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeRoussel Uclaf
InventorsHerman Slijkhuis, Eric Bastiaan Smaal, Gerardus Cornelis Maria Selten
Estimating the term…
An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17.alpha.-hydroxy-progesterone; the conversion of 17.alpha.-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.
An expression cassette comprising a heterologous DNA encoding two or more enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone wherein one of the enzymes catalyzes the conversion of pregnenolone to progesterone and the remaining one or more enzymes catalyze at least one reaction selected from the group consisting of:
A recombinant host cell and progeny thereof comprising a heterologous DNA encoding two or more enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone wherein one of the enzymes catalyzes the conversion of pregnenolone to progesterone and the remaining one or more enzymes catalyze at least one reaction selected from the group consisting of:
An expression cassette comprising aheterologous DNA encoding an enzyme from the metabolic pathway for the bio conversion of cholesterol to hydrocortisone, which enzyme catalyzes the conversion of pregnenolone to progesterone, and further comprising one or more additional heterologous DNAs encoding one or more additional enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone, which one or more additional enzymes catalyze at least one reaction selected from the group consisting of:
Dependencies are inferred from the claim text.