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AssigneeLaboratory of Molecular Biophotonics
InventorsKiyohito Shimura, Kenichi Kasai, Hiroyuki Matsumoto, Hisayoshi Takamoto
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Provided are isoelectric point(pI) markers for isoelectric focusing with fluorescence detection. The markers are fluorescence-labeled oligopeptides which comprise a fluorescence dye bonded chemically to the amino group of N-terminal amino acid of oligopeptide. The marker shows its unique and narrow pI band(peak) in electrophoresis or isoelectric focusing. The markers can be designed to have appropriate pI value, and cover wide range of pI (3<pI<11). Further, the markers have good storage stability. The markers can be preferably applied to the capillary isoelectric focusing with fluorescence detection due to their sharp and narrow band with strong emitted fluorescence.
An isoelectric point marker for isoelectric focusing with fluorescence detection, which is an oligopeptide selected from the group consisting of Asp-His-His-Arg (SEQ ID NO:17), Arg-Asp-Asp-Asp-Asp-Asp (SEQ ID NO:27), Glu-His-Arg-Arg-Arg (SEQ ID NO:31), Glu-His-His-Lys-Arg-Arg (SEQ ID NO:33), Glu-Tyr-Tyr-His-His-Lys-Arg-Arg-Arg (SEQ ID NO:34), Glu-His-His-Lys-Lys-Arg (SEQ ID NO:35), Asp-Tyr-His-Lys-Arg-Arg (SEQ ID NO:36), Asp-Glu-Tyr-His-Lys-Arg-Arg-Arg (SEQ ID NO:37), Asp-Tyr-Tyr-His-Lys-Arg-Arg (SEQ ID NO:38), Asp-Glu-Tyr-Tyr-His-His-Lys-Arg-Arg-Arg (SEQ ID NO:39), Glu-His-His-Arg-Arg (SEQ ID NO:40), Asp-Glu-Tyr-Tyr-Tyr-His-His-Lys-Lys-Arg-Arg (SEQ ID NO:41), Glu-His-His-Lys-Arg (SEQ ID NO:42), Asp-Glu-Tyr-His-His-Arg-Arg-Arg (SEQ ID NO:43), Tyr-Tyr-His-His-His-Arg (SEQ ID NO:44), Asp-Glu-Tyr-His-Arg-Arg-Arg (SEQ ID NO:45), Tyr-His-Arg, Tyr-Tyr-Tyr-His-His-His-Arg (SEQ ID NO:46), Tyr-Tyr-His-His-Arg (SEQ ID NO:7), Glu-Tyr-His-Lys-Arg (SEQ ID NO:48), Asp-Asp-Tyr-His-Lys-Arg-Arg (SEQ ID NO:49), Asp-Tyr-His-Lys-Arg (SEQ ID NO:50), Tyr-His-Lys, Tyr-Tyr-Tyr-His-His-Arg (SEQ ID NO:51), Tyr-Tyr-His-Arg (SEQ ID NO:52), Tyr-Tyr-Tyr-His-Arg (SEQ ID NO:54), Asp-His-His-His-His-Arg (SEQ ID NO:56), Asp-Glu-His-His-Arg-Arg (SEQ ID NO:59), Asp-Asp-Glu-Tyr-His-His-Lys-Arg-Arg (SEQ ID NO:60), Tyr-Tyr-Tyr-Arg (SEQ ID NO:61), Asp-Asp-Glu-Tyr-His-Lys-Arg-Arg (SEQ ID NO:62), Asp-Asp-Asp-Glu-Lys-Arg-Arg-Arg (SEQ ID NO:63), Asp-Asp-Asp-Asp-His-His-Arg-Arg (SEQ ID NO:64), Asp-Glu-Arg-Arg (SEQ ID NO:65), Asp-Glu-Glu-Lys-Arg (SEQ ID NO:66), and Asp-Asp-Glu-Arg (SEQ ID NO:67);
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