Leucyl tRNA synthetase polynucleotides of streptococcus
AssigneeSmithKline Beecham Corporation
InventorElizabeth Jane Lawlor
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Abstract
The invention provides leuS polypeptides and DNA (RNA) encoding leuS polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing leuS polypeptides to screen for antibacterial compounds.
Claims28
An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:
- (a) a polynucleotide having at least a 95% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of the reference sequence of SEQ ID NO:2;
- (b) a polynucleotide having at least a 97% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of the reference sequence of SEQ ID NO:2;
- (c) a polynucleotide encoding a polypeptide comprising the amino acid sequence of the reference sequence of SEQ ID NO:2;
- (d) a polynucleotide hybridizing under stringent conditions to a polynucleotide encoding a polypeptide comprising the amino acid sequence of the reference sequence of SEQ ID NO:2;
- (e) a polynucleotide having at least a 95% identity to a polynucleotide encoding the same mature polypeptide expressed by the leucyl tRNA synthetase gene contained in NCIMB Deposit No. 40794;
- (f) a polynucleotide having at least a 97% identity to a polynucleotide encoding the same mature polypeptide expressed by the leucyl tRNA synthetase gene contained in NCIMB Deposit No. 40794;
- (g) a polynucleotide encoding the same mature polypeptide expressed by the leucyl tRNA synthetase gene contained in NCIMB Deposit No. 40794;
- (i) a polynucleotide which is complementary to the polynucleotide of (a), (b), (c), (d), (e), (f) or (g).
An isolated polynucleotide comprising the polynucleotide sequence set forth in SEQ ID NO:1.
An isolated polynucleotide comprising the nucleotides 1 to 2499 set forth in SEQ ID NO:1.
An isolated polynucleotide comprising the polynucleotide sequence set forth in SEQ ID NO:1 or 3.
An isolated polynucleotide consisting of the polynucleotide sequence set forth in SEQ ID NO:1 or 3.
An isolated polynucleotide consisting of the polynucleotide sequence encoding the polypeptide sequence set forth in SEQ ID NO:2 or 4.
An isolated polynucleotide comprising a DNA sequence obtained by screening an appropriate library containing the complete gene encoding an amino acid sequence set forth in SEQ ID NO:2 under stringent hybridization conditions with a probe having a polynucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2 or a fragment thereof, which fragment retains binding and/or catalytic activity;
- and isolating said DNA sequence.
Dependencies are inferred from the claim text.