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AssigneeNovo Nordisk A/S
InventorsEsper Boel, Tove Christensen, Helle Fabbicius Woldike, Ida Birgitte Huge-Jensen
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A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.
A process for expression of a protein product in Aspergillus oryzae comprising the steps of:
An Aspergillus oryzae strain transformed with a recombinant DNA cloning vector system comprising:
An Aspergillus which has been transformed with a recombinant DNA cloning vector system which integrates into the genome of an Aspergillus host, the vector system comprising:
A process for the expression of a protein product in Aspergillus comprising the steps of:
An isolated promoter for expression of a protein product in Aspergillus, comprising the TAKA-amylase promoter, optionally preceded by upstream activating sequences.
A process for producing a protein comprising:
Dependencies are inferred from the claim text.