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AssigneeStryker Corporation
InventorsHermann Oppermann, Engin Ozkaynak, Thangavel Kuberasampath, David C. Rueger, Roy H. L. Pang
Estimating the term…
Disclosed are (1) osteogenic devices comprising a matrix containing substantially pure natural-sourced mammalian osteogenic protein; (2) DNA and amino acid sequences for novel polypeptide chains useful as subunits of dimeric osteogenic proteins; (3) vectors carrying sequences encoding these novel polypeptide chains and host cells transfected with these vectors; (4) methods of producing these polypeptide chains using recombinant DNA technology; (5) antibodies specific for these novel polypeptide chains; (6) osteogenic devices comprising these recombinantly produced proteins in association with an appropriate carrier matrix; and (7) methods of using the osteogenic devices to mimic the natural course of endochondral bone formation in mammals.
An isolated DNA sequence wherein said DNA sequence encodes at least an osteogenically active region of hOP1-PP (residues 1-431 of Seq. ID No. 1);
An isolated nucleic acid having a DNA sequence encoding a polypeptide chain, said polypeptide chain having a amino acid sequence sufficiently duplicative of the sequence comprising residues 335 to 431 of Seq. ID No. 1 such that, said polypeptide chain, when disulfide bonded to a second polypeptide chain, produces a dimeric species having a conformation capable of inducing cartilage and endochondral bone formation in a mammal when disposed within a matrix and implanted in said mammal.
An isolated nucleic acid having a DNA sequence capable of hybridizing to a nucleic acid comprising the nucleotide sequence defined by base pairs 1051 to 1341 of Seq. ID No. 1 and encoding a polypeptide chain that, when disulfide bonded to a second polypeptide chain, produces a dimeric species having a conformation capable of inducing cartilage and endochondral bone formation in a mammal when disposed within a matrix and implanted in said mammal.
An isolated nucleic acid having a DNA sequence encoding a polypeptide chain, said polypeptide chain selected from the group consisting of:
An isolated nucleic acid having a DNA sequence encoding a polypeptide chain, said polypeptide chain having an amino acid sequence sufficiently duplicative of the amino acid sequence described by residues 335 to 431 of Seq. ID No. 1, such that the oxidation of two of said polypeptide chains produces a dimeric species capable of inducing cartilage and endochondral bone formation in a mammal when disposed within a matrix and implanted in said mammal.
An isolated nucleic acid having a DNA sequence sufficiently duplicative of the DNA sequence of Seq. ID No. 1 or Seq. ID No. 3 and encoding a polypeptide chain such that, when said polypeptide chain is oxidized produces a disulfide bonded dimeric species, the dimeric species having a conformation capable of inducing endochondral bone and cartilage formation in a mammal when disposed within a matrix and implanted in said mammal.
An isolated nucleic acid having a DNA sequence encoding a polypeptide chain which can be used as a subunit of a dimeric osteogenic protein, said polypeptide chain having an amino acid sequence sharing greater than 74% identity with the sequence described by amino acid residues 264-402 of Seq. ID No. 28 (hOP2-Ala) such that, said polypeptide chain, when disulfide bonded to a second polypeptide chain produces a dimeric species capable of inducing endochondral bone formation when implanted in a mammal in association with a matrix.
An isolated nucleic acid having a DNA sequence which can hybridize under stringent hybridization conditions to the complementary strand of a nucleic acid encoding the pro region of mOP2-PP (residues 17-260 of Seq. ID No. 26) or hOP2-PP (residues 17-263 of Seq. ID No. 28) and encoding a polypeptide chain which can be used as a subunit of a dimeric osteogenic protein, said dimeric protein having a conformation capable of inducing endochondral bone formation in a mammal when implanted in a mammal in association with a matrix.
An isolated DNA sequence encoding a protein capable of inducing endochondral bone formation in a mammal, said DNA sequence encoding an amino acid sequence selected from the group consisting of:
An isolated DNA sequence which encodes an amino acid sequence comprising
An isolated DNA sequence which encodes an amino acid sequence comprising
An isolated DNA sequence encoding prepro- or pro-OP1.
A process for producing an active osteogenic composition comprising the step of truncating mature OP1 protein.
Mature OP1 protein as secreted from a mammalian host cell upon or after expression of the sequence shown in Seq. ID No. 1.
Dependencies are inferred from the claim text.