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US 5,861,487 AGranted
Genetic sequences encoding flavonoid pathway enzymes and uses therefor
AssigneeInternational Flower Developments Pty. Ltd.
InventorsTimothy Albert Holton, Edwina Cecily Cornish, Filippa Kovacic, Yoshikazu Tanaka, Diane Ruth Lester
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Abstract
The present invention relates to a nucleic acid isolate comprising a sequence of nucleotides encoding, or complementary to a sequence encoding, a dihydrokaempferol (DHK) hydroxylating enzyme or derivative or part thereof. The present invention also relates to transgenic plants carrying and expressing the above mentioned nucleic acid material.
Claims9
An isolated and purified 3',5'-hydroxylase enzyme capable of hydroxylating dihydrokaempferol.
A method of producing a 3',5'-hydroxylase capable of hydroxylating DHK which comprises:
- (a) transforming a suitable host cell with an expression vector comprising an isolated nucleic acid encoding said 3',5'-hydroxylase;
- (b) culturing said host cells under conditions in which said cells express said isolated nucleic acid and produce said 3',5'-hydroxylase;
- and
- (c) recovering said 3',5'-hydroxylase.
1 more element
Dependencies are inferred from the claim text.