Monoclonal antibodies for detecting Norwalk virus
AssigneeUniversity of Massachusetts
InventorsJohn E. Herrmann, Neil R. Blacklow
Life of this patent
Estimating the term…
Abstract
Monoclonal antibodies and their use in assays to determine the presence of Norwalk virus, Norwalk virus particles, or Norwalk virus-specific IgM, in a biological sample. One assay includes the steps of contacting the sample with a monoclonal antibody that specifically binds to Norwalk virus particles in human stool; reacting the sample and the antibody for a time and under conditions that allow the formation of an immunocomplex between the antibody and any Norwalk virus in the sample; and detecting the immunocomplex, the presence of the immunocomplex indicating the presence of human Norwalk virus in the sample. Another assay is an IgM capture antibody immunoassay.
Claims12
An assay to determine the presence of Norwalk virus or Norwalk virus antigens in a biological sample, the assay comprising the steps of
- contacting the sample with monoclonal antibody 1C9 or 1D8, which specifically binds to Norwalk virus particles from a naturally occurring Norwalk virus outbreak in human stool;
- reacting the sample and the monoclonal antibody for a time and under conditions that allow the formation of an immunocomplex between the monoclonal antibody and any Norwalk virus or virus antigens in the sample;
- and
- detecting the immunocomplex, the presence of the immunocomplex indicating the presence of human Norwalk virus or virus antigens in the sample.
An assay to determine the presence of Norwalk virus-specific IgM in a biological sample, the assay comprising the steps of
- isolating IgM in the sample;
- contacting the isolated IgM with purified Norwalk virus or Norwalk virus particles for a time and under conditions that allow the formation of a Norwalk virus-IgM complex between any Norwalk virus-specific IgM in the sample and the purified Norwalk virus or Norwalk virus particles;
- contacting the Norwalk virus-IgM complex with monoclonal antibody 1D8 or 1C9, which specifically binds to Norwalk virus particles from a naturally occurring Norwalk virus outbreak in human stool;
- reacting the complex and the monoclonal antibody for a time and under conditions that allow the formation of an immunocomplex between the monoclonal antibody and any Norwalk virus-IgM complexes;
- and
- detecting the immunocomplex, the presence of the immunocomplex indicating the presence of Norwalk virus-specific IgM in the sample.
A monoclonal antibody that specifically binds to a Norwalk virus antigen in stool from a human patient infected with Norwalk virus from a naturally occurring Norwalk virus outbreak, wherein the monoclonal antibody is 1C9.
A monoclonal antibody that specifically binds to a Norwalk virus antigen in stool from a human patient infected with Norwalk virus from a naturally occurring Norwalk virus outbreak, wherein the monoclonal antibody is 1D8.
Dependencies are inferred from the claim text.