Prf protein and nucleic acid sequences: compositions and methods for plant pathogen resistance
AssigneeThe Regents of the University of California
InventorsBrian S. Staskawicz, Giles Edward Oldroyd, John M. Salmeron, Caius Rommens
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Abstract
The Prf gene of tomato has been cloned and analyzed. Prf encodes a protein with leucine-rich repeat, nucleotide binding, and leucine zipper motifs, identifying it as a member of the resistance gene class that includes RPS2, RPM1, N and L6. When expressed in transgenic plants, Prf confers Fenthion sensitivity and resistance to a wide variety of phytopathogens, including not only Pseudomonas syringae but also unrelated pathogens such as Xanthomonas campestris.
Claims25
An isolated nucleic acid molecule encoding a protein comprising an amino acid sequence as shown in SEQ ID NO:
- 3.
An oligonucleotide comprising at least 20 contiguous nucleotides of a sequence selected from the group consisting of SEQ ID NOS:
- 1 and 2.
An oligonucleotide comprising at least 20 contiguous nucleotides of SEQ ID NO:2.
An isolated nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) the sequence set forth in SEQ ID NO:3;
- and
- (b) a sequence that differs from the sequence set forth in SEQ ID NO:3 by one or more conservative amino acid substitutions, and wherein the polypeptide has Prf biological activity.
An isolated nucleic acid molecule that:
- (a) hybridizes with a nucleic acid probe comprising nucleotides 3150-4494 of SEQ ID NO:1 under wash conditions of 65.degree. C., 0.5XSSC and 0.5% SDS for 1 hour;
- and
- (b) encodes a protein having Prf biological activity.
An isolated nucleic acid molecule that:
- (a) hybridizes with a nucleic acid probe comprising the sequence shown in SEQ ID NO:2 under wash conditions of 65.degree. C., 0.5XSSC and 0.5% SDS for 1 hour;
- and
- (b) encodes a protein having Prf biological activity.
An isolated nucleic acid molecule encoding a tomato Prf protein.
Dependencies are inferred from the claim text.