Crucifer AFT proteins and uses thereof
AssigneeThe General Hospital Corporation
InventorsHong Zhang, Howard M. Goodman
Life of this patent
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Abstract
Purified DNA encoding crucifer AFT proteins and chimeric transcriptional activator proteins from such DNA are disclosed. Such proteins are also involved in plant defense mechanisms by interacting with proteins involved in protecting plants from pathogens. The recombinant polypeptides and fragments are useful in methods of modulating plant gene expression.
Claims17
A transgenic plant containing a transgene encoding an amino acid sequence having at least 90% amino acid identity to the amino acid sequence of the AFT1 polypeptide shown in FIG. 1 (SEQ ID NO:
- 2) and having the same biological activity as said AFT1 polypeptide, operably linked to a constitutive or regulated promoter.
Substantially pure DNA encoding an AFT1 polypeptide having at least 90% amino acid identity to the amino acid sequence of the AFT1 polypeptide shown in FIG. 1 (SEQ ID NO:
- 2) and having the same biological activity as said AFT1 polypeptide.
Substantially pure DNA encoding a recombinant polypeptide comprising an amino acid sequence having at least 90% amino acid identity to the amino acid sequence of the AFT1 polypeptide shown in FIG. 1 (SEQ ID NO:
- 2) and having the same biological activity as said AFT1 polypeptide.
A vector comprising an amino acid sequence having at least 90% amino acid identity to the amino acid sequence of the AFT1 polypeptide shown in FIG. 1 (SEQ ID NO:
- 2) and having the same biological activity as said AFT1 polypeptide, said vector being capable of directing expression of the polypeptide encoded by said DNA in a vector-containing cell.
A transgenic plant which contains substantially pure DNA encoding an amino acid sequence having at least 90% amino acid identity to the amino acid sequence of the AFT1 polypeptide shown in FIG. 1 (SEQ ID NO:
- 2) and having the same biological activity as said AFT1 polypeptide.
A transgenic plant containing the substantially pure DNA encoding a recombinant polypeptide comprising an amino acid sequence having at least 90% amino acid identity to the amino acid sequence of the AFT1 polypeptide shown in FIG. 1 (SEQ ID NO:
- 2) and having the same biological activity as said AFT1 polypeptide.
Dependencies are inferred from the claim text.