Recombinant sweet protein mabinlin
AssigneeUniversity of Hawaii
InventorsSamuel S. M. Sun, Liwen Xiong, Zhong Hu, Hang Chen
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Abstract
Recombinant materials for the production of practical amounts of the sweet protein, mabinlin are provided. In addition, transgenic plants which have inherently sweetened edible parts result from modifying native plants containing edible parts to express the mabinlin gene. Single-chain forms of this protein which retain their sweetening property are also provided.
Claims19
A purified and isolated DNA molecule comprising a first nucleotide sequence that encodes the amino acid sequence of a mabinlin protein subunit A, and a second nucleotide sequence that encodes the amino acid sequence of a mabinlin protein subunit B, from which can be expressed a mabinlin protein having sweet activity.
A recombinant expression system capable, when inserted into a host cell, of expressing mabinlin protein having sweet activity, which system comprises a DNA molecule including a first encoding sequence corresponding to a mabinlin protein subunit A, and a second encoding sequence corresponding to a mabinlin protein subunit B, that are operably linked to control sequences effective in said host cell.
A composition comprising nucleic acid molecules containing a Capparis sequence encoding mabinlin protein, substantially free of nucleic acid molecules not containing said Capparis sequence, wherein said sequence is selected from the group consisting of:
- (a.sub.1) the cDNA for mabinlin isoform MBL-I or the A or B subunit thereof, (SEQ ID NO:3);
- (a.sub.2) the cDNA for mabinlin isoform MBL-II or the A or B subunit thereof, (SEQ ID NO:1);
- (a.sub.3) the cDNA for mabinlin isoform MBL-III or the A or B subunit thereof, (SEQ ID NO:5);
- (a.sub.4) the cDNA for mabinlin isoform MBL-IV or the A or B subunit thereof, (MBL-IV subset of SEQ ID NO:5);
- (b) a nucleotide sequence complementary to (a.sub.1), (a.sub.2), (a.sub.3) or (a.sub.4);
- (c) a fragment of (a.sub.1), (a.sub.2), (a.sub.3) or (a.sub.4), or a nucleotide sequence complementary thereto, that is at least 15 base pairs in length;
- and
- (d) a nucleotide sequence that comprises a fragment (c) and which will selectively hybridize with (a.sub.1), (a.sub.2), (a.sub.3) or (a.sub.4), or a nucleotide sequence complementary thereto, under stringent conditions.
A composition of DNA molecules that consists of DNA molecules comprising the nucleotide sequence complementary to that of the cDNA for mabinlin isoform MBL-I (SEQ ID NO:3), the cDNA for mabinlin isoform MBL-II (SEQ ID NO:
- 1), the cDNA for mabinlin isoform MBL-III (SEQ ID NO:5), or the cDNA for mabinlin isoform MBL-IV (MBL-IV subset of SEQ ID NO:5), or a portion of any thereof containing at least 15 consecutive nucleotides.
A single chain mabinlin protein free of components that accompany it in Capparis masaikai.
Dependencies are inferred from the claim text.