Plants and processes for obtaining them
AssigneesZeneca Limited, University of Miami
InventorsPeter Lewis Keeling, Joseph Lomako, Dave Gieowar-Singh, George William Singletary, William Joseph Whelan
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Abstract
Plants with an altered starch synthesizing ability are produced by incorporating into the genome of the plant at least one donor gene encoding a starch primer. The starch primer is an enzyme capable of initiating starch synthesis, such as an amylogenin and/or glycogenin. DNA constructs encoding a starch primer are provided, particularly constructs encoding amylogenin from maize.
Claims22
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having amylogenin activity wherein said polypeptide is encoded by a plant gene and wherein said nucleotide sequence hybridizes at 65.degree. C. in 5.times.SSC to a DNA probe selected from the group consisting of:
- a DNA having the complement of the sequence in Table 4 (SEQ ID NO:15), a DNA having the complement of the sequence in Table 5 (SEQ ID NO:16) and a DNA having the complement of the sequence encoding the polypeptide having amylogenin activity of the maize cDNA clone contained in the deposit at the American Type Culture Collection under the accession number ATCC 69389.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having amylogenin activity wherein said polypeptide is encoded by a maize gene.
A plant or progeny thereof each stably transformed by a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having glycogenin activity wherein said polypeptide is encoded by an animal gene.
Dependencies are inferred from the claim text.