Fungus-responsive chimaeric gene
AssigneeMax-Planck-Gesellschaft zur Forderung
InventorsGunter Strittmatter, Norbert Martini
Life of this patent
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Abstract
Fungus-responsive chimaeric genes are used in the production of transgenic plants which can produce plant cells surrounding the fungus infection that become capable of killing, disabling or repelling the fungus or that are themselves killed or rendered unsuitable for the fungus to feed upon, thereby preventing the spread of the fungus infection.
Claims34
A plant transformed with a first and a second foreign DNA sequence which, when expressed, inhibits sporulation and spread of a fungus infection said plant, said first foreign DNA sequence comprising:
- (a) a fungus-responsive promoter region whose action in controlling transcription of a DNA sequence is stimulated by fungal infection in those plant cells immediately surrounding the site of fungal infection, operably linked to
- (b) a region encoding a RNA, a protein or a polypeptide which when expressed in plant cells, kills said plant cells;
- and said second foreign DNA sequence comprising:
- (a) a constitutive plant-expressible promoter, operably linked to
- (b) a region encoding a RNA, a protein or a polypeptide which inhibits or inactivates the first foreign DNA sequence or its encoded product in plant cells not immediately surrounding said site of fungal infection.
A method for preventing or delaying sporulation of a pathogenic fungus on a plant, comprising the step of:
- providing two foreign DNA sequences into the cells of said plant, wherein said first foreign DNA sequence comprises:
- (a) a fungus-responsive promoter region whose action in controlling transcription of a DNA sequence is stimulated by fungal infection in those plant cells immediately surrounding the site of fungal infection, operably linked to
- (b) a region encoding a RNA, a protein or a polypeptide which when expressed in plant cells, kills said plant cells;
- and wherein said second foreign DNA sequence comprises:
- (a) a constitutive plant-expressible promoter, operably liked to
- (b) a region encoding a RNA, a protein or a polypeptide which can inhibit or inactivate the first foreign DNA sequence or its encoded product.
A plant transformed with a first and second foreign DNA sequence which, when expressed, inhibits sporulation and spread of a fungus infecting said plant, said first foreign DNA sequence comprising:
- (a) a fungus-responsive promoter region whose action in controlling transcription of a DNA sequence is stimulated by fungal infection in those plant cells immediately surrounding the site of fungal infection, operably linked to
- (b) a region encoding a RNA, a protein or polypeptide which when expressed in plant cells, kills or disturbs the functioning of said plant cells;
- and said second foreign DNA sequence comprising:
- (a) a constitutive plant-expressible promoter, operably linked to
- (b) a region encoding a RNA, a protein or a polypeptide which inhibits or inactivates the first foreign DNA sequence or its encoded product in plant cells not immediately surrounding said site of fungal infection.
A method for preventing or delaying sporulation of a pathogenic fungus on a plant, comprising the step of:
- providing two foreign DNA sequences into the cells of said plant, wherein said first foreign DNA sequence comprises:
- (a) a fungus-responsive promoter region whose action in controlling transcription of a DNA sequence is stimulated by fungal infection in those plant cells immediately surrounding the site of fungal infection, operably linked to
- (b) a region encoding a RNA, a protein or a polypeptide which when expressed in plant cells, kills or disturbs the functioning of said plant cells;
- and wherein said second foreign DNA sequence comprises:
- (a) a constitutive plant-expressible promoter, operably lined to
- (b) a region encoding a RNA, a protein or a polypeptide which can inhibit or inactivate the first foreign DNA sequence or its encoded product.
Dependencies are inferred from the claim text.