Genetic sequences encoding flavonol synthase enzymes and uses therefor
AssigneeInternational Flower Developments Pty. Ltd.
InventorsTimothy Albert Holton, Lisa Ann Keam
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Abstract
The present invention relates generally to genetic sequences encoding flavonoid pathway metabolising enzymes and in particular enzymes having flavonol synthase activity and their use such as in manipulating production of pigmentory molecules in plants. More particularly, the present invention provides genetic sequences encoding flavonol synthase (FLS).
Claims43
An isolated nucleic acid encoding or complementary to a sequence encoding a flavonol synthase (FLS), said sequence encoding a FLS comprising a nucleotide sequence as set forth in at least one of SEQ ID NOs:1-5 or a nucleotide sequence having at least 50% similarity to at least one of SEQ ID NOs:1-5.
An isolated DNA molecule comprising a sequence of nucleotides which
- (i) encodes a FLS of plant origin;
- and
- (ii) has at least 50% nucleotide sequence similarity to the sequence set forth in SEQ ID No:1.
An isolated nucleic acid molecule which:
- (i) encodes a FLS of plant origin;
- and
- (ii) hybridizes under low stringency conditions to the nucleotide sequence set forth in SEQ ID NO:1 or to a complementary strand thereof.
A transgenic plant carrying a non-indigenous genetic sequence encoding a FLS.
A transgenic plant consisting of at least one of petunia, rose, carnation, chrysanthemum, gerbera, tobacco, lisianthus, lily, iris or pelargonium carrying a non-indigenous genetic sequence encoding a FLS, said genetic sequence capable of being expressed in said plant and wherein said FLS is encoded by a DNA molecule comprising a DNA strand capable of hybridising under low stringency conditions to a nucleic acid molecule comprising the sequence of nucleotides set forth in at least one of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5 or a nucleotide sequence having at least 50% sequence similarity to at least one of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5.
Dependencies are inferred from the claim text.