Isolated DNA elements that direct pistil-specific and anther-specific gene expression and methods of using same
AssigneeCornell Research Foundation, Inc.
InventorsMikhail E. Nasrallah, June B. Nasrallah, Mary K. Thorsness
Life of this patent
Estimating the term…
Abstract
Isolated DNA elements that direct either pistil-specific or anther-specific expression of a polypeptide-encoding gene are disclosed. Plants comprising the DNA fragment operably linked to a gene are also disclosed. A further embodiment comprises a method of producing a female sterile plant, the method comprising growing a plant having integrated into its genome an isolated DNA element that directs pistil-specific expression of a nucleotide sequence that encodes or is transcribed into a moiety that causes female sterility in plants.
Claims21
An isolated DNA element that directs expression of an operably linked coding sequence of a gene comprising a sequence selected from the -339 to -79 region or the -339 to -143 region of the SLG.sub.13 promoter
- wherein said DNA element is linked to a minimal promoter.
A chimeric gene comprising a DNA element comprising SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 operably linked to a coding sequence of at least one gene that encodes a polypeptide or RNA,
- wherein said coding sequence does not naturally occur in operable linkage with said DNA element, and
- said DNA element directs pistil-specific expression of said coding sequence in either a 5' or 3' or a 3' to 5' orientation with respect to the coding sequence, wherein said DNA element is linked to a mimimal promoter.
A chimeric gene comprising a DNA element of the SLG13 promoter from the -339 to -79 region or from -339 to -143 region operably linked to a coding sequence of a gene that encodes a polypeptide or RNA,
- wherein said coding sequence does not naturally occur in operable linkage with said DNA element, and
- said DNA element directs pistil-specific expression of said coding sequence in either a 5' or 3' or a 3' to 5' orientation with respect to the coding sequence.
Dependencies are inferred from the claim text.