Transgenic non-human animals having targeting endogenous lymphocyte transduction genes and cognate human transgenes
AssigneeThe Regents of the University of California
InventorsDaniel Littman, Shinichiro Sawada, Nigel Killeen
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Abstract
The invention provides transgenic non-human animals and transgenic non-human mammalian cells having at least one functionally disrupted lymphocyte transduction locus, particularly a CD4 locus, targeting constructs used to produce such transgenic stem cells and animals, methods and targeting constructs for inactivating or suppressing expression of endogenous lymphocyte transduction gene loci, transgenes encoding heterologous lymphocyte transduction proteins, and nonhuman animals that express a human lymphocyte transduction protein and lack expression of a cognate murine lymphocyte transduction protein.
Claims8
A transgenic mouse comprising a diploid genome having a functionally disrupted endogenous CD4 gene, wherein said disruption is in the nucleotide sequence of one or more coding region exons, and having integrated into its genome a gene encoding human CD4, wherein said human CD4 gene is transcriptionally regulated by an operably linked human CD4 promoter and upstream T cell specific enhancer and wherein said mouse expresses human CD4 protein in a T cell specific manner.
Dependencies are inferred from the claim text.