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US 5,859,231 AGranted
Synthesis of oligonucleotides with boranophosphonate linkages
AssigneeDuke University
InventorsBarbara Ramsay Shaw, Kenneth W. Porter, Dmitri Sergueev
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Abstract
The present invention relates, in general, to a process of enzymatically synthesizing nucleic acids containing boranophosphonate linkages that are resistant to degradation. The invention further relates to methods of utilizing such nucleic acids in DNA and RNA amplification and sequencing, gene therapy and molecular detection protocols.
Claims8
A method of synthesizing an oligonucleotide comprising an internucleotide boranophosphate linkage comprising:
- i) synthesizing an oligonucleotide comprising an internucleotide H-phosphonate diester linkage,
- ii) reacting said oligonucleotide resulting from step (i) with an agent that converts said H-phosphonate diester linkage to a phosphite triester linkage, under conditions such that said conversion is effected,
- iii) boronating said phosphite triester linkage resulting from step (ii) so that a boranophosphate triester linkage is formed;
- and
- iv) subjecting said boranophosphate triester linkage resulting from step (iii) to conditions such that said boranophosphate triester linkage is converted to a boranophosphate diester linkage so that said oligonucleotide comprising an internucleotide boranophosphate linkage is formed.
2 more elements
Dependencies are inferred from the claim text.