Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
AssigneeNeXstar Pharmaceuticals, Inc.
InventorsNebojsa Janjic, Larry Gold, Paul Schmidt
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Abstract
This invention discloses a method for preparing a complex comprised of a VEGF Nucleic Acid Ligand and a Lipophilic Compound by identifying a VEGF Nucleic Acid Ligand by SELEX methodology and associating the VEGF Nucleic Acid Ligand with a Lipophilic Compound. The invention further discloses Complexes comprising one or more VEGF Nucleic Acid Ligands in association with a Lipophilic Compound. The invention further includes a Lipid construct comprising a VEGF Nucleic Acid Ligand or Complex and methods for making the same.
Claims7
A method for the preparation of a Complex comprised of a vascular endothelial growth factor (VEGF) Nucleic Acid Ligand and a Lipophilic Compound, said method comprising:
- a) contacting the Candidate Mixture with VEGF, wherein Nucleic Acids having an increased affinity to VEGF relative to the Candidate Mixture may be partitioned from the remainder of the Candidate Mixture;
- b) partitioning the increased affinity VEGF Nucleic Acids from the remainder of the Candidate Mixture;
- c) amplifying the increased affinity VEGF Nuclcic Acids to yield a ligand-enriched mixture of Nucleic Acids;
- and
- d) covalently linking said identified VEGF Nucleic Acid Ligand with a Lipophilic Compound.
A method for preparing a Lipid Construct comprising a Complex comprised of a VEGF Nucleic Acid Ligand and a Lipophilic Compound, said method comprising:
- a) contacting the Candidate Mixture with VEGF, wherein Nucleic Acids having an increased affinity to VEGF relative to the Candidate Mixture may be partitioned from the rermainider of the Candidate Mixture;
- b) partitioning the increased affinity VEGF Nucleic Acids from the remnainder of the Candidate Mixture;
- c) amplifying the increased affinity VEGF Nucleic Acids to yield a ligand-enriched mixture of Nucleic Acids;
- d) covalently linking said identified VEGF Nucleic Acid Ligand with a Lipophilic Compound to form a Complex;
- and
- e) forming a Lipid Construct comprising said Complex.
Dependencies are inferred from the claim text.