Factors for cellular functional regulation and biological applications thereof
AssigneeCabinet Armengaud Aine
InventorsChristiane Guillouzo, Anne Corlu, Bernard Kneip
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Abstract
The invention relates to proteins characterized in that they are proteins for communication between cells of a given type and the contiguous or proximal heterologous cells.
Claims11
An isolated and substantially purified protein having the following properties:
- specifically reacts with a monoclonal antibody secreted by a clone having deposit number DSM ACC 2011,
- mediates cell-cell interactions,
- contains at least one peptide selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6,
- is expressed in at least one mammalian tissue selected from the group consisting of the non-parenchymatous cells of liver, epithelial cell of the biliary ductules, endothelial cell, Ito cell and macrophage exocrine pancreas, testicles, a Sertoli cell of the seminiferous tubule and a spermatocyte between the eptotene stage and zygotene stage, ovaries, a mature ovocyte and follicular cells surrounding a mature ovocyte, hematopoietic tissue, bone marrow, stromal tissue of bone marrow, thymus, lymph node and spleen, myocyte, granulocyte, erythrocyte and lymphocyte, and
- is a glycoprotein with a single amino acid chain appearing as a dominant band with a molecular weight of about 85,000 daltons, or of about 73,000 daltons, as measured by SDS polyacrylamide gel electrophoresis, and having a pI of about 4.9 to 5.1 or having a pI of about 5.2, respectively.
An isolated nucleic acid molecule consisting of a nucleotide sequence encoding a peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5.
An isolated nucleic acid molecule consisting of a nucleotide sequence encoding a peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:6, SEQ ID NO:3 and SEQ ID NO:7.
An isolated peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5.
An isolated peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:6, SEQ ID NO:3, and SEQ ID NO:7.
An isolated and substantially purified protein produced by the process comprising the steps of:
- solubilizing mammalian cell membranes to form a mixture, said mammalian cell membranes being obtained from a mammalian tissue selected from the group consisting of the non-parenchymatous cells of liver, epithelial cell of the biliary ductules, endothelial cell, Ito cell and macrophage, exocrine pancreas, testicles, a Sertoli cell of the seminiferous tubule and a spermatocyte between the leptotene stage and zygotene stage, ovaries, a mature ovocyte and follicular cells surrounding a mature ovocyte, hematopoietic tissue, bone marrow, stromal tissue of bone marrow, thymus, lymph node and spleen, myocyte, granulocyte, erythrocyte and lymphocyte,
- contacting said solubilized membranes with a monoclonal antibody secreted by a clone having deposit number DSM ACC 2011 under conditions whereby said antibody will specifically bind to a protein present in said solubilized membranes to form an antibody-protein conjugate,
- separating said conjugate from said mixture,
- separating said antibody from said conjugate to produce said protein;
- said protein being capable of mediating cell-cell interactions;
- and
- said protein contains at least one peptide selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6.
Dependencies are inferred from the claim text.