Vaccines comprising nucleotide sequences encoding bovine herpesvirus type 1 g1, g111 and gIV
AssigneeUniversity of Saskatchewan
InventorsLorne Babiuk, Sylvia van der Hurk, Tim Zamb, David Fitzpatrick
Life of this patent
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Abstract
Recombinant subunit vaccines against bovine herpesvirus type 1 (BHV-1) are provided, as well as methods of vaccination and methods of recombinantly producing the subunit antigens or nucleotide sequences employed in the vaccines.
Claims12
A vaccine composition to prevent or ameliorate the symptoms of disease comprising an isolated nucleotide sequence encoding a polypeptide containing at least one protective determinant of a BHV-1 polypeptide, operably linked to one or more control sequences such that said isolated nucleotide sequence is expressed in a host cell, the polypeptide being selected from the group consisting of gI, wherein said isolated nucleotide sequence comprises the contiguous nucleotide sequence depicted in FIG. 5;
- gIII, wherein said isolated nucleotide sequence comprises the contiguous nucleotide sequence depicted in FIG. 6;
- and gIV, wherein said isolated nucleotide sequence comprises the contiguous nucleotide sequence depicted in FIG. 7.
A vaccine composition to prevent or ameliorate the symptoms of disease comprising an isolated nucleotide sequence encoding a polypeptide containing at least one protective determinant of a recombinant BHV-1 glycoprotein, operably linked to one or more control sequences such that said isolated nucleotide sequence is expressed in a host cell, said recombinant BHV-1 glycoprotein selected from the group consisting of BHV-1 gI having an unglycosylated molecular weight of about 105 kDa and encoded by the nucleotide sequence as depicted in FIG. 5;
- gIII glycoprotein having a molecular weight of about 91 kDa and encoded by the nucleotide sequence as depicted in FIG. 6;
- and a gIV glycoprotein having a molecular weight of about 71 kDa and encoded by the nucleotide sequence as depicted in FIG. 7.
A vaccine composition to prevent or ameliorate the symptoms of disease comprising an isolated nucleotide sequence encoding a polypeptide containing at least one protective determinant of a truncated BHV-1 gIV polypeptide operably linked to one or more control sequences such that said isolated nucleotide sequence is expressed in a host cell, wherein said polypeptide consists of residues 1-355 as depicted in FIG. 5.
Dependencies are inferred from the claim text.