Back to results
US 5,858,784 AGranted
Expression of cloned genes in the lung by aerosol- and liposome-based delivery
AssigneeThe Regents of the University of California
InventorsRobert James Debs, Ning Zhu
Life of this patent
Estimating the term…
Abstract
Methods and compositions for producing a mammal capable of expressing an exogenously supplied gene in cells of the airway are disclaimed. Liposome-nucleic acid complexes are prepared then delivered via aerosol to the lung airway. The invention provides a direct method for transforming pulmonary cells as a means for treating disorders of the lung as for providing a means for delivering substances systematically following expression in the lung.
Claims14
A composition comprising:
- an aerosolized transfection agent, prepared by aerosolizing a mixture comprising complexes between DNA molecules comprising expression cassettes and cationic lipid carriers in a pharmaceutically acceptable carrier, wherein the cationic lipid carriers are in the form of vesicles having diameter of less than 5 .mu.m, wherein said expression cassettes and said cationic lipid carriers are present in said mixture in a ratio ranging from about 4:1 to about 1:2 mg DNA to micromole of cationic lipid, wherein said mixture is substantially free of macroaggregates of said complexes.
Dependencies are inferred from the claim text.