Production of normal mammalian organ culture using a medium containing mem-alpha, leibovitz L-15, glucose galactose fructose
AssigneeThe United States of America as represented by the Administrator of the National Aeronautics and Space Administration
InventorsThomas J. Goodwin, David A. Wolf, Glenn F. Spaulding, Tacey L. Prewett
Life of this patent
Estimating the term…
Abstract
Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under micro-gravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated micro-gravity created in a horizontal rotating wall culture vessel. The medium used for culturing the cells, especially a mixture of epithelial and mesenchymal cells contains a mixture of Mem-alpha and Leibovits L15 supplemented with glucose, galactose and fructose.
Claims6
A process for producing functional non-neoplastic mammalian organ tissue comprising culturing the mammalian organ tissue in a culture medium, consisting essentially of:
- (a) Minimal Essential Medium-alpha (MEM-alpha) medium
- (b) Leibovitz L-15 medium;
- (c) tri-sugar supplement comprising glucose, galactose, and fructose;
- (d) serum;
- (e) NaHCO.sub.3 ;
- (f) HEPES;
- (g) folic acid;
- (h) nicotinic acid;
- (i) bactopeptone;
- (j) I-inositol;
- (k) L-glutamine;
- (l) gentamycin;
- (m) funguizone;
- and
- (n) insulin-transferrin-sodium selenite.
Dependencies are inferred from the claim text.