Regulatory nucleotide sequence of the initiation of transcription
AssigneeInstitut Pasteur
InventorsPhilippe Mazodier, Gerard Guglielmi
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Abstract
The invention relates to a recombinant nucleotide sequence, characterized in that it comprises: a regulatory sequence of the initiation of transcription, this regulatory sequence containing a promoter in association with the motif GCACTC 9N GAGTGC, in which "N" signifies any one of the 4 bases thymine, guanine, adenine and cytosine; a sequence coding for a polypeptide, called "heterologous polypeptide", which is different from that naturally associated with the promoter; the coding sequence being positioned downstream from the regulatory sequence of the initiation of transcription at a site which, under suitable conditions, would allow the expression of the polypeptide under the control of the promoter.
Claims16
A recombinant nucleotide sequence comprising:
- a regulatory sequence for initiation of transcription, this regulatory sequence including a promoter contained in the SmaI-SmaI fragment of pPM1005 or in the BgIII-SstI fragment of pPM997, operably linked to;
- a sequence coding for a heterologous polypeptide different from that naturally associated with said promoter, wherein said coding sequence is positioned downstream from said regulatory sequence for initiation of transcription at a site which, under suitable conditions, allows the polypeptide to be expressed under the control of said promoter.
A heat shock polypeptide comprising:
- either (i) one of the amino acid sequences (SEQ ID NOs:8 and 10) shown below;
- or (ii) a part of this sequence (i), said part comprising the NH.sub.2 terminus and said polypeptide having a molecular weight of about 18 kDa and an isoelectric point of about 9:
- SEQ ID NO:
- 8:
- ##STR6## and SEQ ID NO:
- 10:
- ##STR7##
An isolated nucleotide sequence selected from the following sequences:
- P1 corresponding to one of the sequences (SEQ ID Nos:
- 2-3):
- ##STR8## P2 corresponding to one of the sequences (SEQ ID Nos. 4-5):
- ##STR9##
An 18 kD heat shock protein isolated from Streptomyces albus and having an isoelectric point higher than 9.
Dependencies are inferred from the claim text.