Cell culture plate with oxygen and carbon dioxide-permeable waterproof sealing membrane
AssigneeBrandeis University
InventorDaniel Perlman
Life of this patent
Estimating the term…
Abstract
A cell culture plate which contains cells incubated in a liquid medium held in sample wells formed and arranged over the surface of the plate, is covered and sealed with a waterproof adhesive sealing membrane which excludes microbial contaminants, but which is functionally permeable to oxygen and carbon dioxide gases. The membrane allows cellular respiration to continue, with the transmission rate of oxygen and carbon dioxide through the membrane being essentially uniform from well to well over the surface of the plate.
Claims11
A cell culture plate comprising one or more cells held within a liquid medium contained in a plurality of wells formed and arranged within said plate, wherein said wells are covered and sealed with a waterproof adhesive sealing membrane which excludes microbial contaminants, and is permeable to oxygen and carbon dioxide gases, and wherein a first lower surface of said sealing membrane is coated with a non-cytotoxic pressure-sensitive adhesive material which adheres said sealing membrane to said culture plate.
A kit comprising a culture plate comprising sample wells formed and arranged within said culture plate, and a sterile sheet assembly comprising a sized portion of the waterproof sealing membrane which excludes microbial contaminants, and is permeable to oxygen and carbon dioxide gases, and which comprises a first lower surface which is coated with a non-cytotoxic pressure-sensitive adhesive material for attaching said membrane to said culture plate.
Dependencies are inferred from the claim text.