Delivery of nucleic acids
AssigneeMedical Research Council
InventorsStephen James Russell, Francois-Loic Cosset, Frances Joanne Morling, Robin Anthony Weiss, Mary Katharine L. Collins
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Abstract
The invention provides a recombinant viral particle for delivering a nucleic acid to mammalian cells, the particle comprising an MLV-env protein that binds to an MLV-env receptor expressed on the surface of a target cell so as to cause infection thereof, and a surface-exposed EGF that binds to an EGF receptor generally not expressed on the surface of the target cell, but expressed on non-target cells that also express the MLV-env receptor, such that binding of the viral particle to the EGF receptor via EGF inhibits infection of the non-target cell by the viral particle.
Claims15
A method of restricting the host range of a viral particle, said viral particle comprising an MLV-env protein that binds to an MLV-env receptor expressed on the surface of a target cell so as to cause infection thereof, comprising introducing onto the surface of said viral particle a an EGF that binds to an EGF receptor molecule expressed on the surface of a non-target cell of the population of cells which are hosts for infection by the virus, such that binding of said viral particle to said EGF receptor via said EGF inhibits infection of said non-target cell by said viral particle.
A recombinant viral particle for delivering a nucleic acid to mammalian cells, said particle comprising an MLV-env protein that binds to an MLV-env receptor expressed on the surface of a target cell so as to cause infection thereof, and a surface-exposed EGF that binds to an EGF receptor generally not expressed on the surface of said target cell, but expressed on non-target cells that also express said MLV-env receptor, such that binding of said viral particle to said EGF receptor via said EGF inhibits infection of said non-target cell by the viral particle.
A method of extending the host range of a viral particle comprising a nucleic acid for delivery to a target cell, the method comprising introducing onto the surface of said viral particle an EGF that binds to an EGF receptor, said binding not generally facilitating infection of the target cell by said viral particle, administering said particle to a target cell comprising an EGF receptor, and administering an inhibitor of lysosomal acidification.
Dependencies are inferred from the claim text.