Method to stabilize cell suspensions using aged heavy metal solution and paraformaldehyde
AssigneeNorthern General Hospital N.H.S. Trust
InventorsVivian Granger, David Barnett
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Abstract
A stabilized cell suspension is prepared by treating the cell suspension with a solution of a heavy metal compound, which has been aged at pH 6.5-7.5 prior to use to allow a precipitate to form, removing the precipitate from the heavy metal solution, mixing the aged heavy metal solution with the cell suspension to form a first mixture, and mixing the first mixture with a paraformaldehyde solution to form a stabilized cell suspension which is still capable of lysis.
Claims23
A method of treating a suspension of cells capable of lysis comprising the steps of:
- preparing an aqueous solution of a heavy metal compound which has been aged at a pH of from 6.5 to 7.5 for at least 24 hours to allow precipitate to form;
- removing the precipitate from the solution to form an aged aqueous solution of the heavy metal compound, the aged aqueous solution comprising less than 1% weight per volume (w/v) of the heavy metal compound;
- mixing the cell suspension and the aged solution of the heavy metal compound at a pH of from 6.5 to 7.5 to form a first mixture;
- and,
- mixing the first mixture with a paraformaldehyde solution comprising up to 1% weight per volume (w/v) paraformaldehyde at a pH of from 6.5 to 7.5 to form a stabilised cell suspension which is still capable of lysis.
A method of treating a suspension of cells capable of lysis comprising the steps of:
- preparing a 0.1%-0.5% aqueous solution of a heavy metal compound which has been aged at pH 6.5 to 7.5 for at least one week, which is an amount of time sufficient to allow any precipitate to form;
- removing the precipitate from the solution to form an aged aqueous solution of the heavy metal compound;
- mixing the cell suspension and the aged solution of the heavy metal compound at pH 6.5 to 7.5 and at a temperature of 0.degree. C. to 8.degree. C. to form a first mixture;
- and,
- mixing the first mixture with a solution comprising 0.1%-0.5% paraformaldehyde at a pH of 6.5 to 7.5 and at a temperature of 0.degree. C. to 8.degree. C. to form a stabilized cell suspension which is still capable of lysis.
Dependencies are inferred from the claim text.