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US 5,858,698 AGranted
Methods for detection of enteropathogenic e. coli
AssigneeUniversity of Alberta
InventorsGlen D. Armstrong, Rosa P. Vanmaele
Life of this patent
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Abstract
Therapeutic and diagnostic methods are provided for treatment and detection of enteropathogenic E. coli (EPEC) enteric infections. Also provided is an assay for the identification of compositions which are effective inhibitors of EPEC infection.
Claims9
A method for detecting the possible presence of enteropathogenic E. coli in a sample comprising:
- a. contacting a monolayer of epithelial cells with the sample suspected of containing enteropathogenic E. coli, under conditions and for sufficient time to allow the E. coli to bind to and infect the cells;
- and
- b. detecting the presence of enteropathogenic E. coli by measuring the disruption of the monolayer.
A method for detecting the possible presence of enteropathogenic E. coli in a sample suspected of containing enteropathogenic E. coli comprising:
- a. contacting an isomer of lactosamine or sialyllactosamine with the sample;
- and
- b. detecting the binding of bacteria to the isomer, wherein binding of bacteria to the isomer indicates the possible presence of enteropathogenic E. coli.
Dependencies are inferred from the claim text.