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US 5,858,696 AGranted
Method and compositions to assess oxidative stress in vivo
AssigneeVanderbilt University
InventorsL. Jackson Roberts, II, Jason D. Morrow
Life of this patent
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Abstract
This invention relates to a method to assess oxidative stress in vivo by quantification of prostaglandin F.sub.2 -like compounds and their metabolites produced by a noncyclooxygenase free radical catalyzed mechanism.
Claims10
A method to assess oxidative stress in vivo comprising:
- (a) subjecting phospholipids in a biological sample to reverse phase solid chromatography extraction using a C-18 cartridge,
- (b) eluting prostanoids retained on the C-18 cartridge with a 80:20:0.1 mixture of water/acetonitrile/acetic acid;
- (c) maintaining the eluate of a phospholipids from a fresh sample of lipid containing biological fluid at -20.degree. C.;
- (d) measuring an amount of noncyclooxygenase derived prostanoids present in the eluate of the phospholipids from the fresh sample of lipid containing biological fluid maintained at -20.degree. C.;
- (e) comparing said measured amount of prostanoids with a control;
- and
- (f) assessing oxidative stress in vivo based on the comparison in step (b).
4 more elements
A method to assess oxidative stress in vivo comprising:
- (a) measuring the amount of noncyclooxygenase derived prostanoids in a biological sample before the ex vivo development of prostanoids in the sample;
- (b) comparing the measured amount of the noncyclooxygenase derived prostanoids with a control;
- and (c) assessing oxidative stress in vivo based on the comparison in step (b wherein said prostanoids are selected from the group consisting of:
- ##STR5##
1 more element
A method to assess oxidative stress in vivo comprising:
- (a) measuring the amount of noncyclooxygenase derived prostanoids in a biological sample before the ex vivo development of prostanoids in the sample;
- (b) comparing the measured amount of the noncyclooxygenase derived prostanoids with a control;
- and
- (c) assessing oxidative stress in vivo based on the comparison in step (b) wherein said prostanoids are selected from the group consisting of:
- ##STR6##
2 more elements
A method to assess oxidative stress in vivo comprising:
- (a) measuring the amount of noncyclooxygenase derived prostanoids in a biological sample before the ex vivo development of prostanoids in the sample;
- (b) comparing the measured amount of the noncyclooxygenase derived prostanoids with a control;
- and
- (c) assessing oxidative stress in vivo based on the comparison in step (b) wherein said prostanoid comprises 2,3-dinor-5,6-dihydro-8-iso-prostaglandin F.sub.2.alpha..
1 more element
A purified and isolated compound of the formula 2,3-dinor-5,6-dihydro-8-iso-prostaglandin F.sub.2.alpha..
Dependencies are inferred from the claim text.