Polychlorinated biphenyls (PCB) immunoassay method, its components and a kit for use in performing the same
AssigneeEnsys, Inc.
InventorsStephen B. Friedman, William B. Studabaker, Randy L. Allen
Life of this patent
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Abstract
An immunoassay method that integrates a sample processing component that enables the testing of a variety of environmental matrixes and the components for performing the method, wherein the immunoassay utilizes a monoclonal anti-PCB antibody to detect the presence or absence of PCB based contamination in a sample when tested in a field or laboratory location.
Claims26
A monoclonal antibody useful for detecting the presence of PCB's in a sample, having specific reactivity towards AROCLORs 1260, 1254, 1248, 1242, 1232, 1016 and 1221 that is substantially the same as the monoclonal antibody produced by clone ATCC No. HB-12421.
An immunoassay kit useful for detecting the presence of PCB's in a sample, comprising a monoclonal antibody having specific reactivity towards AROCLORs 1260, 1254, 1248, 1242, 1232, 1016 and 1221 that is substantially the same as the monoclonal antibody produced by clone ATCC No. HB-12421.
An immunoassay kit useful for detecting the presence of PCB's in a sample, said kit comprising
- (i) a reaction vessel means containing an immobilized monoclonal antibody having specific reactivity towards AROCLORs 1260, 1254, 1248, 1242, 1232, 1016 and 1221 that is substantially the same as the monoclonal antibody produced by clone ATCC No. HB-12421,
- (ii) a standard solution comprising at least one of AROCLOR 1260, AROCLOR 1254 or AROCLOR 1248, and a solvent;
- (iii) a hapten-reporter moiety-containing reagent which is cross-reactive with said antibody, and a suitable solvent;
- and
- (iv) means for causing said reporter moiety to generate a signal and means for detecting the signal.
The kit of claim 5, wherein said hapten-reporter moiety-containing reagent is a compound of formula ##STR12## wherein X and Y independently represent a halogen n is an integer from 0 to 5,
- m is an integer from 0 to 4, wherein n and m cannot both be 0;
- R.sub.5 is ##STR13## or a single bond R.sub.6 is ##STR14## wherein R.sub.7 is hydrogen, C.sub.1 -C.sub.2 alkyls, linear, branched, or cyclic C.sub.3 -C.sub.6 alkyls;
- and
- p is O or an integer from 1 to 4;
- linked to a reporter molecule.
An immunoassay for determining the presence of PCB's in a sample, comprising:
- (i) combining (ia) a monoclonal antibody with specific reactivity towards AROCLORs 1260, 1254, 1248, 1242, 1232, 1016 and 1221 that is substantially the same as the monoclonal antibody produced by clone ATCC No. HB-12421, with (ib) a mixture of (ibi) the sample and (ibii) a hapten-and-reporter-molecule reagent which is cross reactive with said monoclonal antibody, wherein said reporter molecule is susceptible to producing a detectable signal, to form an assay mixture;
- (ii) incubating said assay mixture to allow competitive monoclonal antibody binding between at least one of said AROCLORs, if present, in the sample, and said reagent;
- (iii) causing production of said detectable signal and correlating said signal to the amount of reagent bound to said monoclonal antibody to obtain a measure of the amount of PCB's in said sample.
A monoclonal antibody useful for detecting the presence of PCB's in a sample, wherein said monoclonal antibody is a monoclonal antibody produced by clone ATCC No. HB-12421.
An immunoassay kit useful for detecting the presence of PCB's in a sample, comprising a monoclonal antibody produced by clone ATCC No. HB-12421.
An immunoassay kit useful for detecting the presence of PCB's in a sample, said kit comprising
- (i) a reaction vessel means containing an immobilized monoclonal antibody wherein the immobilized monoclonal antibody is a monoclonal antibody produced by clone ATCC No. HB-12421,
- (ii) a standard solution comprising at least one of AROCLOR 1260, AROCLOR 1254 or AROCLOR 1248, and a solvent;
- (iii) a hapten-reporter moiety-containing reagent which is cross-reactive with said antibody, and a suitable solvent;
- and
- (iv) means for causing said reporter moiety to generate a signal and means for detecting the signal.
An immunoassay for determining the presence of PCB's in a sample, comprising:
- (i) combining (ia) a monoclonal antibody produced by clone ATCC No. HB-12421, with (ib) a mixture of (ibi) the sample and (ibii) a hapten-and-reporter-molecule reagent which is cross reactive with said monoclonal antibody, wherein said reporter molecule is susceptible to producing a detectable signal, to form an assay mixture;
- (ii) incubating said assay mixture to allow competitive monoclonal antibody binding between at least one of said AROCLORs, if present, in the sample, and said reagent;
- (iii) causing production of said detectable signal and correlating said signal to the amount of reagent bound to said monoclonal antibody to obtain a measure of the amount of PCB's in said sample.
Dependencies are inferred from the claim text.