Method for determining the presence of functional p53 by measuring GADD45 protein expression
OwnerAlbert J. Fornace, Jr., Michael B. Kastan +1inventors
InventorsAlbert J. Fornace, Jr., Michael B. Kastan, France Carrier
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Abstract
The dependence of ionizing radiation-induced GADD45 mRNA and protein expression on the presence of functional p53 in mammalian cells is disclosed. First and second oligonucleotide sequences are provided which can form a double-stranded oligomer capable of binding to functional p53 protein. The present invention demonstrates that the dependence of ionizing radiation-induced GADD45 mRNA and protein expression on the presence of functional p53 and the binding of functional p53 to a double-stranded oligomer binding sequence can serve as the bases for methods for determining the presence of functional p53 in mammalian cell lines and tumors.
Claims6
A method for determining the presence of functional p53 in mammalian cells by measuring GADD45 protein expression, comprising the steps of:
- (a) stimulating the mammalian cells to increase GADD45 protein expression by irradiating said cells with ionizing radiation or with a radiomimetic compound in an amount sufficient to stimulate GADD45 protein expression;
- and
- (b) comparing the level of GADD45 protein in said stimulated cells to the level of GADD45 protein in unstimulated cells.
Dependencies are inferred from the claim text.