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US 5,858,677 AGranted
Protein D--an IgD-binding protein of haemophilus influenzae
OwnerArne Forsgreninventor
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OwnerArne Forsgreninventor
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A novel surface exposed protein of H. influenzae or related Haemophilus species is described. The protein named protein D is an Ig receptor for human IgD and has an apparent molecular weight of 42,000. Protein D can be detected in all of 116 encapsulated and non-encapsulated isolates of H. influenzae studied. The protein from all strains shows in addition to the same apparent molecular weight immunogenic similarities since protein D from all strains interacts with three different mouse monoclonal antibodies and monoclonal human IgD. A method for purification of protein D is described. Cloning of the protein D gene from H. influenzae in E. coli is described as well as the nucleotide sequence and the deduced amino acid sequence corresponding to a molecular weight of 41,821 daltons including a putative signal sequence of 18 amino acids containing a consensus sequence (SEQ ID NO: 2), Leu-Ala-Gly-Lys for bacterial lipoproteins.
A method of detecting the presence of Haemophilus influenzae or related Haemophilus species in a sample by contacting said sample with a DNA probe or primer constructed to specifically correspond to the nucleic acids which code for protein D of Haemophilus influenzae or related Haemophilus species, said protein having an apparent molecular weight of 42,000 as determined by SDS-PAGE under reducing conditions, a capacity of binding IgD and being encoded by the nucleic acid sequence of SEQ ID NO:
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