Nucleic acid fragments and corresponding peptide fragments from the caprine arthritis-encephalitis virus (CAEV) genome, and uses thereof
AssigneeCentre National De La Recherche Scientifique
InventorsGiuseppe Bertoni, Gianfranco Pancino, Ernst Peterhans, Pierre Sonigo
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Abstract
Nucleotide fragments from caprine arthritis-encephalitis virus (CAEV) env gene, corresponding peptide fragments, and uses thereof in screening for viral caprine arthritis-encephalitis, are disclosed. Antibodies to peptide fragments and uses thereof for diagnosing viral arthritis-encephalitis are also enclosed. Said nucleic acid fragments code for a peptide fragment including at least one CAEV Env protein segment comprising at least one immunodominant epitope selected from the transmembrane region of said protein, and include 15-255 nucleotides.
Claims15
A nucleic acid fragment encoding a peptide fragment,
- wherein the peptide fragment contains at least one immunodominant epitope of the transmembrane region of the CAEV Env protein;
- wherein the nucleic acid fragment comprises a sequence selected from the group consisting of SEQ ID NO:1, the sequence corresponding to positions 8019-8264 of the gene encoding the CAEV Env protein, the sequence corresponding to positions 7991-8107 of the gene encoding the CAEV Env protein, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:7;
- wherein SEQ ID NO:1 encodes the G1 peptide fragment, the sequence corresponding to positions 8019-8264 encodes the G2 peptide fragment, the sequence corresponding to positions 7991-8107 encodes the G3 peptide fragment, SEQ ID NO:2 encodes the G4 peptide fragment, SEQ ID NO:3 encodes the G5 peptide fragment, SEQ ID NO:4 encodes the TM1 peptide fragment, SEQ ID NO:6 encodes the TM3 peptide fragment, and SEQ ID NO:7 encodes the TM4 peptide fragment.
A peptide fragment,
- wherein the peptide fragment contains at least one immunodominant epitope of the transmembrane region of the CAEV Env protein;
- wherein the peptide fragment comprises a sequence selected from the group consisting of the sequence corresponding to positions 665-717 of the CAEV Env protein, the sequence corresponding to positions 670-751 of the CAEV Env protein, the sequence corresponding to positions 661-698 of the CAEV Env protein, the sequence corresponding to positions 732-783 of the CAEV Env protein, the sequence corresponding to positions 694-749 of the CAEV Env protein, the sequence corresponding to positions 680-694 of the CAEV Env protein, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:
- 11,
- wherein positions 1, 9, and 10 of SEQ ID NO:10 are each His or Gin, and position 13 of SEQ ID NO:10 is Ile or Val,
- wherein the sequence corresponding to positions 665-717 comprises the G1 peptide fragment, the sequence corresponding to positions 670-751 comprises the G2 peptide fragment, the sequence corresponding to positions 661-698 comprises the G3 peptide fragment, the sequence corresponding to positions 732-783 comprises the G4 peptide fragment, the sequence corresponding to positions 694-749 comprises the G5 peptide fragment, the sequence corresponding to positions 680-694 comprises the TM1 peptide fragment, SEQ ID NO:8 comprises the TM3 peptide fragment, SEQ ID NO:
- 10 comprises the G1 peptide fragment, and SEQ ID NO:11 comprises the TM4 peptide fragment.
Dependencies are inferred from the claim text.