Method for the rapid and ultra-sensitive detection of leukemic cells
AssigneeLife Technologies Corporation
InventorsPaul E. Nisson, Nicoletta Sacchi
Life of this patent
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Abstract
An improved method is disclosed for diagnosing the presence of a chromosomal translocation characteristic of acute myelogenous leukemia. Nucleic acid molecules that may be used in this improved method are described.
Claims27
A method of screening for the presence of a leukemic cell in a sample comprising:
- a) obtaining RNA from said sample;
- b) contacting said RNA in the presence of at least two nucleic acid molecules, wherein a first of said nucleic acid molecules is capable of hybridizing to a nucleotide sequence of chromosome 8 of a non-leukemic cell comprising SEO ID NO:17 and a second of said nucleic acid molecules is capable of hybridizing to an AML1 sequence of chromosome 21 of a non-leukemic cell;
- and
- c) determining whether said RNA is capable of hybridizing to both said first and said second nucleic acid molecules.
A method of screening for the presence of a leukemic cell in a sample comprising:
- a) obtaining cDNA from said sample;
- b) contacting said cDNA with at least two nucleic acid molecules, wherein a first of said nucleic acid molecules is capable of hybridizing to a nucleotide sequence of chromosome 8 of a non-leukemia cell comprising SEO ID NO:17 and a second of said nucleic acid molecules is capable of hybridizing to an AML1 sequence of chromosome 21 of a non-leukemia cell;
- and
- c) determining whether said cDNA is capable of hybridizing to both said first and said second nucleic acid molecules.
An isolated RNA or cDNA molecule comprising a nucleotide sequence of chromosome 8 of a non-leukemic cell which contains SEQ ID NO:17 and an AML1 nucleotide sequence of chromosome 21 of a non-leukemic cell.
A method of screening for the presence of a leukemic cell in a sample comprising:
- a) obtaining cDNA from said sample;
- and
- b) amplifying said cDNA in the presence of at least two nucleic acid molecules, wherein said amplified cDNA molecule is capable of hybridizing to a nucleotide sequence of chromosome 8 of a non-leukemic cell comprising SEQ ID NO:
- 17 and an AML1 sequence of chromosome 21 of a non-leukemic cell.
Dependencies are inferred from the claim text.