Method of quantitating genomic DNA

A biological product substantially free of foreign heteroqenous genomic DNA, said qenomic DNA comprising repetitive sequences, as determined by a method of quantitating heteroqenous genomic DNA in a sample comprising:

• adding to a sample comprising the product a given amount of at least one nucleic acid as an internal standard, said at least one standard nucleic acid differing from said repetitive sequences in at least one detectable characteristic,
• amplifying said repetitive sequences and said at least one standard nucleic acid with a nucleic acid amplification process employing primers complementary to said repetitive sequences, and
• determining, as a first amount, the amount of amplified repetitive sequences, and, as at least one second amount, the amount of the at least one amplified standard nucleic acid, and
• determining from said first amount and said at least one second amount, as a third amount, the amount of total heterogenous genomic DNA originally contained in said sample,
• wherein said biological product is substantially free from foreign heteroqenous genomic DNA if said third amount is less than 10 to 100 pg per dose.

A kit for quantitating genomic nucleic acids in a sample comprising;

• at least one known nucleic acid which differs from the nucleic acids to be quantitated in at least one detectable characteristic, as an internal standard,
• fluorescence-labelled primers binding to repetitive genomic sequences,
• a positive control comprising a known amount of genomic nucleic acid, and
• a negative control comprising a buffer free of nucleic acids.

A kit for the quantitation of genomic primate DNA in a samples, comprising:

• as an internal standard, plasmid pAlu20
• fluorescence-labelled primers Alu A2/2 and AluB
• a positive control comprising a known amount of Vero cell DNA and
• a negative control comprising a buffer free of genomic nucleic acid.

A kit for the quantitation of genomic avian DNA in a sample, comprising:

• as an internal standard, plasmids pCR1+15 and pCR1-8
• fluorescence-labelled primers CR1 and CR1A
• a positive control comprising a known amount of avian DNA and
• a negative control comprising a buffer free of genomic nucleic acid.