Assay utilizing hydrogen peroxide adduct
AssigneeMochida Pharmaceutical Co., Ltd.
InventorsKeiko Kuzuya, Tadakazu Yamauchi
Life of this patent
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Abstract
Improvement in assays utilizing at least hydrogen peroxide for one analysis reagent is provided. The assay of the present invention employs a stable hydrogen peroxide adduct in dry state which has no adverse effects on the assay, and which has a high hydrogen peroxide-retaining ability. In the assay, an aqueous solution is added to an adduct in dry state of (a) at least one member selected from the group consisting of a carboxylic acid and a salt thereof, phosphoric acid and a salt thereof, and a sulfonic acid and a salt thereof, and (b) hydrogen peroxide to generate peroxide, and the thus generated peroxide is used for the analysis reagent.
Claims17
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of monocarboxylic acids represented by the following formula (1):
- ##STR6## wherein ##STR7## pyruvic acid, vanillic acid, picolinic acid and sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of an aliphatic dicarboxylic acid, an aromatic dicarboxylic acid, and sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of tricarboxylic acids represented by the following formula (2):
- ##STR8## wherein R.sup.4 is H or --OH, and p is 1 to 3, q is 0 to 3, and r is 0 to 3;
- and formula (3):
- ##STR9## wherein R.sup.5 is H or CH.sub.3, and s is from 1 to 3;
- and sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of tetracarboxylic acids represented by the following formula (4):
- ##STR10## wherein t is from 0 to 3, u is from 0 to 3, and v is from 0 to 3;
- sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of a uronic acid, a polyuronic acid, and sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of a phosphoric acid, and sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of a hydroxyalkanesulfonic acid, aminoalkanesulfonic acid, hydroxybenzenesulfonic acid, and chondroitin sulfate, and sodium and potassium salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of a monosaccharide, a disaccharide, a sugar alcohol, and polymers thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an anaysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of a N-acetylglucosamine, ascorbic acid, creatinine, and polyethylene glycol;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
A process for generating peroxide in the course of an assay wherein at least hydrogen peroxide is used as an analysis reagent, wherein the improvement comprises the steps of:
- (1) preparing an adduct in a dry state of
- (a) at least one member selected from the group consisting of a solid substance containing carboxyl group, phosphono group, sulfo group, and salts thereof;
- and
- (b) hydrogen peroxide;
- and
- (2) adding an aqueous solution to said adduct in a dry state in the course of the analysis to thereby generate peroxide as an analysis reagent in the assay.
Dependencies are inferred from the claim text.