Glycophospholipid
AssigneeSnow Brand Milk Products Co., Ltd.
InventorsMakoto Shiota, Goro Hanagata, Kiyoshi Tatsumi
Life of this patent
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Abstract
The present invention relates to glycophospholipids represented by the general formula I!: ##STR1## The glycophospholipid of the present invention can be obtained by extracting lipid fraction of whey, butter milk, etc. with a mixed solvent of chloroform/methanol and purifying by silica gel chromatography. The glycophospholipid of the present invention is that having a novel structure and existing in milk lipid, and utilized as a biochemical reagent, a starting material for medicine and foodstuff or a surfactant.
Claims6
A purified glycophospholipid isolated from milk or milk a product comprising the following physico-chemical properties (1) to (12):
- (1) has the following proton peaks detected by proton NMR:
- four proton peaks at approximately 4.0 ppm, a proton peak at 5.1 ppm, an anomeric proton peak at 4.2 ppm, a methyl proton peak at 0.9 ppm and a proton peak at 1.5-3.0 ppm;
- (2) has characteristic absorption at about 3,500 cm.sup.-1 , 1,000 cm.sup.-1 and 1,300 cm.sup.-1 as determined by Fourier transformation infrared analysis;
- (3) decomposes when treated with phospholipase C;
- (4) displays a color reaction characteristic of phospholipid when contacted with Dittmer-Lester reagent;
- (5) releases sugar when contacted with .beta.-galactosidase;
- (6) displays a color reaction characteristic of sugar when contacted with orcinol-sulfuric acid, diphenylamine-aniline reagent;
- (7) has two sugar molecules linked via a .beta.(1.fwdarw.6) linkage, wherein the end terminal sugar is galactose;
- (8) is colored when treated with ninhydrin;
- (9) has an ethanolamine moiety according to quantitative analytical determination of nitrogen, carbon and hydrogen;
- (10) has a glycerol moiety according to quantitative analytical determination of carbon and hydrogen;
- (11) has a phosphoric acid group according to quantitative analytical determination of phosphorous;
- and
- (12) has a C.sub.12-20 saturated or unsaturated fatty acid which can be detected by analysis of methyl ester after decomposition when contacted with hydrochloric acid/methanol.
A purified glycophospholipid comprising the following physico-chemical properties (1) to (12):
- (1) has the following proton peaks detected by proton NMR:
- four proton peaks at approximately 4.0 ppm, a proton peak at 5.1 ppm, an anomeric proton peak at 4.2 ppm, a methyl proton peak at 0.9 ppm and a proton peak at 1.5-3.0 ppm;
- (2) has characteristic absorption at about 3,500 cm.sup.-1, 1,000 cm.sup.-1 and 1,300 cm.sup.-1 as determined by Fourier transformation infrared analysis;
- (3) decomposes when treated with phospholipase C;
- (4) displays a color reaction characteristic of phospholipid when contacted with Dittmer-Lester reagent;
- (5) releases sugar when contacted with .beta.-galactosidase;
- (6) displays a color reaction characteristic of sugar when contacted with orcinol-sulfuric acid, diphenylamine-aniline reagent;
- (7) has two molecules of galactose linked together via a .beta.(1.fwdarw.6) linkage;
- (8) is colored when treated with ninhydrin;
- (9) has an ethanolamine moiety according to quantitative analytical determination of nitrogen, carbon and hydrogen;
- (10) has a glycerol moiety according to quantitative analytical determination of carbon and hydrogen;
- (11) has a phosphoric acid group according to quantitative analytical determination of phosphorous;
- and
- (12) has a C.sub.12-20 saturated or unsaturated fatty acid which can be detected by analysis of methyl ester after decomposition when contacted with hydrochloric acid/methanol.
Dependencies are inferred from the claim text.