Molecular cloning and expression of G-protein coupled receptors
OwnerAlexander John MacLennaninventor
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Abstract
The cloning and expression of two novel rat cDNAs ("H218"and "rat-edg") which encode two members ("p.sup.H218 " and "p.sup.rat-edg ") of the G-protein coupled receptor superfamily of proteins is described. The amino acid sequence similarity between "p.sup.H218 " and "p.sup.rat-edg " suggests that they may be activated by the same endogenous ligand(s). The expression pattern of mRNA transcripts of both genes in cell lines, various rat tissues and developing rat brain suggests that they both play a role in cell proliferation and/or differentiation. The polynucleotide molecules, proteins, and antibodies of the subject invention can be used in both diagnostic and therapeutic applications.
Claims5
An isolated polynucleotide molecule which encodes a p.sup.H218 polypeptide, said polynucleotide molecule comprising the nucleotide sequence shown in SEQ ID NO.1, or a polynucleotide molecule which hybridizes to said polynucleotide molecule under stringent hybridization conditions.
An isolated p.sup.H218 polypeptide encoded by a polynucleotide molecule comprising the nucleotide sequence shown in SEQ ID NO:1, or a polynucleotide molecule which hybridizes to said polynucleotide molecule under stringent hybridization conditions.
An isolated p.sup.H218 peptide, wherein said peptide has an amino acid sequence shown in SEQ ID NO:5.
Dependencies are inferred from the claim text.