Compositions comprising complement related proteins and carbohydrates, and methods for producing and using said compositions

A composition comprising a soluble complement receptor protein or functional derivative or analog thereof capable of binding a complement component, and a carbohydrate moiety which is a ligand for a cellular adhesion molecule selected from the group consisting of P-selectin, E-selectin, and L-selectin.

A composition comprising soluble complement receptor type 1 or a functional derivative or analog thereof capable of binding a complement component, and a carbohydrate moiety which is a ligand for a cellular adhesion molecule selected from the group consisting of P-selectin, E-selectin, and L-selectin.

A pharmaceutical composition comprising a soluble complement regulatory protein moiety which has a short consensus repeat structural motif and which binds a complement component, and a carbohydrate moiety which binds a cellular adhesion molecule selected from the group consisting of P-selectin, E-selectin, and L-selectin, dispersed in a pharmaceutically acceptable carrier.

A soluble complement inhibitory protein comprising a selectin ligand.

A method for producing a composition comprising a complement moiety and a carbohydrate moiety, which comprises:

• (a) expressing a complement related protein in a cell which glycosylates the complement related protein with a Lewis X antigen;
• and
• (b) recovering the complement inhibitory protein expressed in step (a).

A method for producing a complement inhibitory protein comprising a selectin ligand, which comprises linking a complement inhibitory protein with a selectin ligand.

A method for treating a subject with a disease or disorder involving complement activity comprising administering to a subject in need of such treatment a complement inhibiting amount of a composition comprising a soluble complement regulatory protein moiety which has a short consensus repeat structural motif and which binds a complement component, and at least one carbohydrate moiety which is a ligand for a cellular adhesion molecule selected from the group consisting of P-selectin, E-selectin and L-selectin.

A method for diagnosing an inflammatory condition comprising the steps of:

• 1) immobilizing a composition comprising a complement moiety and a carbohydrate moiety, and
• 2) incubating said immobilized composition with a sample suspected of containing a ligand for the composition of step (1) so immobilized, and
• 3) detecting the amount of binding that has occurred.

A method for producing a composition that inhibits an activity associated with complement, which comprises:

• (a) expressing a soluble protein moiety having a short consensus repeat motif in a cell which glycosylates said protein moiety with a carbohydrate that is a selectin ligand;
• and
• (b) recovering a complement inhibitory composition from said cell.

A method for treating a subject with an inflammatory condition comprising administering to a subject in need of such treatment an inflammation inhibiting amount of a composition comprising a soluble complement regulatory protein moiety which has a short consensus repeat structural motif and which binds a complement component, linked to a carbohydrate moiety which binds a cellular adhesion molecule selected from the group consisting of P-selectin, E-selectin and L-selectin.