DNA encoding interleukin-4 receptors
AssigneeImmunex Corporation
InventorsBruce Mosley, David J. Cosman, Linda Park, M. Patricia Beckmann, Carl J. March, Rejean Idzerda
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Abstract
Mammalian Interleukin-4 receptor proteins, DNAs and expression vectors encoding mammalian IL-4 receptors, and processes for producing mammalian IL-4 receptors as products of cell culture, are disclosed. A method for suppressing an IL-4-dependent immune or inflammatory response in a mammal, including a human, by administering an effective amount of soluble IL-4 receptor (sIL-4R) and a suitable diluent or carrier.
Claims12
An isolated DNA sequence encoding a biologically active human IL-4 receptor (IL-4R), selected from the groups consisting of:
- (a) CDNA clones that encode a human IL-4R comprising the amino acid sequence of amino acids 1-800 in FIGS. 4A-4C,
- (b) DNA sequences capable of hybridization to the clones of (a) under moderately stringent conditions and which encode biologically active human EL-4R;
- and
- (c) DNA sequences which are degenerate as a result of the genetic code to the DNA sequences define in (a) or (b) and which encode biologically active human IL-4R.
A purified EL-4R polypeptide, comprising a fragment selected from the group consisting of:
- a) an immunogenic fragment of the murine protein of FIGS. 2A-2C and
- b) an immunogenic fragment of the human protein of FIGS. 4A-4C.
A purified polypeptide comprising an amino acid sequence that is at least 80% identical to a sequence selected from the group consisting of:
- a) amino acids 1 to 785 of FIGS. 2A-2C;
- b) amino acids 1 to 800 of FIGS. 4A-4C;
- c) amino acids 1 to 208 of FIG. 2A;
- and
- d) amino acids 1 to 207 of FIG. 4A:
- wherein said polypeptide is a naturally occurring polypeptide that is capable of binding IL-4.
A purified polypeptide, wherein said polypeptide is encoded by a DNA selected from the group consisting of:
- a) a DNA comprising the nucleotide sequence presented as nucleotides 1 to 2355 of FIGS. 2A-2C:
- b) a DNA comprising the nucleotide sequence presented as nucleotides 1 to 2400 of FIGS. 4A-4C:
- and
- c) a DNA that hybridizes under moderately stringent conditions to a DNA of (a) or (b);
- wherein said polypeptide is a naturally occurring polypeptide that is capable of binding IL-4.
An isolated polypeptide selected from the group consisting of:
- a) an immunogenic fragment of the murine protein of FIGS. 2A-2C;
- and
- b) an immunogenic fragment of the human protein of FIGS. 4A-4C.
An isolated polypeptide capable of binding IL-4, selected from the group consisting of the murine polypeptide of FIGS. 2A-2C and the human polypeptide of FIGS. 4A-4C, with the proviso that said polypeptide is devoid of a transmembrane region.
Dependencies are inferred from the claim text.