Back to results
US 5,856,186 AGranted
Method for producing a highly enriched population of osteoclast cells
AssigneeMerck & Co., Inc.
InventorsSevgi B. Rodan, Gregg Wesolowski, Gideon A. Rodan
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeMerck & Co., Inc.
InventorsSevgi B. Rodan, Gregg Wesolowski, Gideon A. Rodan
Estimating the term…
Methods of obtaining enriched populations of osteoclast precursor cells which can be released from tissue culture dishes and used for biochemical studies are described. Osteoblastic cells and bone marrow cells are co-cultured. Next a .alpha..sub.v .beta..sub.3 receptor ligand, such as echistatin is used for cell detachment. The result is an 75-95% pure enriched population of tartrate resistant acid phosphatase (TRAP.sup.+) cells, in high yields (2-3.times.10.sup.6 cells per experiment) can be obtained. These cells are mostly mononucleated and based on their characteristics are considered to be pre-fusion osteoclasts (pOC cells). The precursor osteoclasts can be reseeded onto osteoblasts to obtain an enriched population of mature, multinucleated osteoclast cells.
A method of producing a cell population which is highly enriched with mature mammalian osteoclast cells wherein said population is derived from precursor osteoclast cells which are TRAP.sup.+, comprising:
A population of cells highly enriched in mammalian osteoclasts wherein said population is derived from precursor osteoclast cells which are TRAP.sup.+, comprising:
A suspension culture comprising a population highly enriched of mature mammalian osteoclast cells wherein said population is derived from precursor osteoclast cells which are TRAP.sup.+, produced by a process comprising:
Dependencies are inferred from the claim text.