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US 5,856,172 AGranted
Preservation of microorganisms in a vial with a cap comprising an immobilized desiccant
AssigneeQuality Technologies, LLC
InventorsRick Greenwood, Nathan Greene
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Abstract
The present invention provides methods and compositions for the safe, effective, and efficient storage and transport of microbial cultures. In particular, the present invention provides compositions and methods for the storage and transport of bacterial cultures. The present invention is suitable for use in circumstances where long-term storage of microbial cultures is desired.
Claims11
A method of preserving a sample of microorganisms, comprising:
- a) providing:
- i) lyophilization medium,
- ii) viable microorganisms,
- iii) a vial, and
- iv) a cap, wherein said cap comprises an immobilized desiccant;
- b) exposing said viable microorganisms to said lyophilization medium to produce a lyophilization solution;
- c) dispensing said lyophilization solution into said vial;
- and
- d) lyophilizing said lyophilization solution to produce a preserved culture.
6 more elements
A method of preserving a sample of microorganisms, comprising:
- a) providing:
- i) lyophilization medium comprising one or more cryoprotectants,
- ii) microorganisms,
- iii) a vial, and
- iv) a cap, wherein said cap comprises an immobilized desiccant;
- b) exposing said microorganisms to said lyophilization medium to produce a lyophilization solution;
- c) dispensing said lyophilization solution into said vial;
- d) exposing said lyophilization solution to freeze-drying to produce a preserved culture;
- e) sealing said cap to said vial to produce a vial and cap combination containing said preserved culture;
- f) opening said vial and cap combination;
- and
- g) reviving said preserved microorganisms.
9 more elements
Dependencies are inferred from the claim text.