Structural gene of pneumococcal protein
AssigneeThe UAB Research Foundation
InventorsDavid E. Briles, Janet L. Yother
Life of this patent
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Abstract
A purified pneumococcal surface protein A (PspA) comprises a truncated form of the PspA protein which is immunoprotective and contains the protective epitopes of PspA. The PspA protein is soluble in physiologic solution and lacks at least the cell membrane anchor region of the whole protein. The protein is formed by insertion-duplication of mutagenesis of S. pneumoniae with pspA gene and expression of the truncated protein into the growth medium.
Claims24
An isolated, recombinant DNA molecule coding for a truncated Pneumococcal surface protein (PspA) wherein said truncated form of the PspA contains at least the N-terminal alpha-helical protective epitope containing region of the whole PspA protein and said whole PspA comprises a sequence as set forth in SEQ ID NO:
- 1.
An isolated, recombinant DNA molecule coding for a truncated form of the Pneumococcal surface protein (PspA) protein which contains the immunoprotective epitopes of the protein and contains up to about 90% of the whole PspA protein from which the cell membrane anchor region is absent, said DNA molecule having a coding sequence comprising that set forth in FIG. 3 (SEQ ID NO:
- 1).
A method of forming an in immunoprotective truncated PspA protein, which comprises:
- effecting insertion-duplication mutagenesis of a bacterium with a cloned pspA gene containing an insertion mutation within its coding region to produce a mutated bacterium expressing a truncated PspA protein,
- growing said mutated bacterium to effect expression of a truncated PspA protein, and
- isolating said protein.
Dependencies are inferred from the claim text.