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US 5,856,167 AGranted
Hypochlorite stable protease from bacillus SP., DSM 8473
AssigneeNovo Nordisk A/S
InventorHelle Outtrup
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Abstract
A protease obtained from Bacillus sp., DSM 8473, is disclosed which has improved hypochlorite stability as compared to other known proteases. The protease is suitable as a detergent additive and may be used singly or combined with other know enzymes in detergent compositions. A process for washing soiled fabric with detergent compositions containing the hypochlorite stable protease is also disclosed.
Claims7
A protease derived from the strain Bacillus sp., DSM 8473, having the following properties:
- (a) an apparent molecular weight of approximately 30 kD as determined by SDS-PAGE;
- (b) an isoelectric point of about 8.8 as determined by isoelectric focusing on LKB Ampholine PAG plates;
- (c) optimum activity at a temperature in the range from 50.degree. C. to 60.degree. C., determined at pH 9.5 with casein as substrate;
- (d) more than 80% activity at a pH in the range of 6-11 when measured at 25.degree. C. with casein as substrate;
- and
- (e) a residual activity of at least 80% after 60 minutes at 25.degree. C. in 5 ppm hypochlorite relative to the activity of the protease in the absence of 5 ppm hypochlorite.
3 more elements
Dependencies are inferred from the claim text.