Alkaline cellulase and method of producing same
AssigneeGenencor International, Inc.
InventorPieter Van Solingen
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Abstract
The present invention provides a novel cellulase composition obtainable from Bacillus sp. CBS 670.93. A preferred cellulase has a calculated molecular weight of approximately 50 kD, a calculated isoelectric point of about 4 and a pH optimum on CMC of about 6-10 at 40.degree. C. and about 7 at 60.degree. C.
Claims5
An isolated and purified cellulase obtainable from or derived from Bacillus sp. CBS 670.93, having an amino acid sequence comprising SEQ ID NO:2.
A composition comprising a cellulase which comprises an amino acid sequence according to SEQ ID NO:2.
A cellulase obtainable from Bacillus sp. CBS 670.93 having an amino acid sequence comprising SEQ ID NO:2, wherein said cellulase is produced by a method comprising the following steps:
- (a) transforming a suitable microorganism with DNA encoding said cellulase;
- (b) preparing a fermentation broth containing said suitable microorganism under conditions suitable for expression of said DNA;
- (c) maintaining said fermentation broth for a time and under conditions to permit the expression of a desired amount of said cellulase;
- and
- (d) collecting said fermentation broth which contains said cellulase.
Dependencies are inferred from the claim text.