Tumor necrosis factor receptor-I-associated protein kinase and methods for its use
AssigneeResearch Development Foundation
InventorsBharat B. Aggarwal, Bryant G. Darnay
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Abstract
The present invention provides an isolated and purified protein that associates with the cytoplasmic domain of the p60 form of the tumor necrosis factor receptor, having a molecular weight of about 52-55 kDa on SDS-PAGE, is a phosphoprotein, and does not bind to the p80 form of the tumor necrosis factor receptor. Also provided is an isolated and purified protein kinase that binds to the cytoplasmic domain of the p60 form of the tumor necrosis factor receptor, said kinase phosphorylates the p60 form of the tumor necrosis factor receptor. Also provided are various methods of manipulating this tumor necrosis factor receptor-associated protein and kinase in order to reduce various biological effects of tumor necrosis factor.
Claims8
An enriched and isolated protein kinase that binds to the acidic rich-carboxy terminus of the cytoplasmic domain of the p60 form of the tumor necrosis factor receptor, said kinase phosphorylates the p60 form of the tumor necrosis factor receptor at serine and threonine residues, said kinase exhibits optimal phosphorylation in the presence of Mg2+, said kinase is inducible by tumor necrosis factor.
Dependencies are inferred from the claim text.