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US 5,856,159 AGranted
Production of galactosyltransferase
AssigneeCytel Corporation
InventorCarl Perez
Life of this patent
Estimating the term…
Abstract
A gene expression system for producing galactosyltransferase comprises an NSO cell recombinantly modified with a polynucleotide encoding galactosyltransferase or a portion thereof which catalyzes the reaction: UDP-D-galactose+N-acetylglucosamine.fwdarw.UDP+D-galactosyl-N-acetyl-D-gluc osamine Further provided is a method for producing galactosyltransferase by culturing the gene expression system.
Claims2
A method for producing galactosyltransferase, comprising the steps of:
- (a) culturing a gene expression system comprising the HSO cell having the accession number ATCC No. CRL 12066 which is recombinantly modified with a polynucleotide encoding the galactosyltransferase or an enzymatically active portion thereof which catalyzes the reaction:
- UDP-D-galactose+N-acetylglucosamine.fwdarw.UDP+D-galactosyl-N-acetyl-D-gluc osamine;
- and
- (b) harvesting the galactosyltransferase.
1 more element
A recombinantly modified NSO cell having the accession no. ATCC No. CRL 12066.
Dependencies are inferred from the claim text.