.DELTA..sup.9 14:0-ACP fatty acid desaturase and gene therefor
AssigneeThe Penn State Research Foundation
InventorsRichard Craig, June I. Medford, Ralph O. Mumma, Diana L. Cox-Foster, David Schultz
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Abstract
Isolation and characterization of a novel plant fatty acid desaturase cDNA that encodes a .DELTA..sup.9 14:0-ACP desaturase. Expression of the .DELTA..sup.9 14:0-ACP desaturase is a critical factor for pest resistance in plants of the genus Pelargonium and other plants generally; the desaturase gene is also useful in other contexts and for other purposes such as increasing the percentage of unsaturated fatty acids in oil-producing crops such as soybeans, rapeseed, maize, sunflower, safflower, cotton, cuphea, peanut, coconut and oil-palm, as well as increasing the percentage of unsaturated fatty acids in other plants generally.
Claims7
An isolated and purified DNA nucleotide sequence comprising:
- (a) the nucleotide sequence set forth in SEQ. ID. NO. 1 or
- (b) a nucleotide sequence encoding the polypeptide set forth in SEQ. ID. NO. 2,
- wherein said nucleotide sequence encodes a 14:0-ACP desaturase.
A method of making mRNA encoding a 14:0-ACP desaturase comprising growing a cell or plant having integrated in its genome a DNA sequence having the nucleotide sequence set forth in SEQ. ID. NO. 1 or having a nucleotide sequence encoding the polypeptide set forth in SEQ. ID. NO. 2 under conditions which permit transcription of the 14:0-ACP desaturase encoding sequence.
A nucleic acid vector, comprising one of:
- a) the nucleotide sequence set forth in SEQ ID NO. 1;
- or
- b) a nucleotide sequence encoding the polypeptide set forth in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.