Microbial cholesterol dehydrogenase, process for its production and use
AssigneeBoehringer Mannheim GmbH
InventorsDorothee Ambrosius, Klaus Kaluza, Stephan Gross
Life of this patent
Estimating the term…
Abstract
Oxygen-independent cholesterol-converting enzyme obtainable from Actinomycetes or Basidiomycetes which utilizes artificial electron acceptors as well as a method and reagent for the determination of (total) cholesterol using the enzyme.
Claims7
An isolated, cholesterol converting enzyme having cholesterol dehydrogenase activity, wherein said cholesterol converting enzyme oxidizes cholesterol by removing electrons from cholesterol and transferring said electrons to an artificial electron acceptor, wherein the transfer of said electrons does not require oxygen, said cholesterol converting enzyme having a molecular weight of approximately 55000 Da as determined by SDS-PAGE, wherein said cholesterol converting enzyme is an Actinomycetes or a Basidiomycetes cholesterol converting enzyme.
An isolated, cholesterol converting enzyme having cholesterol dehydrogenase activity, wherein said enzyme oxidizes cholesterol by removing electrons from cholesterol and transferring said electrons to an artificial electron acceptor, wherein the transfer of said electrons does not require oxygen, said isolated cholesterol converting enzyme being obtained from a microorganism selected from the group consisting of Rhodococcus BMTU 3899, DSM 9444, Rhodococcus erythropolis, DSM 743;
- Streptomyces hydgroscopicus, DSM 40771, Flammulina velutipes, DSM 1658;
- Coprinus comatus, DSM 1746;
- and Trametes versicolor BMTU 3107, DSM 9443.
Dependencies are inferred from the claim text.