Materials and methods for biosynthesis of serine and serine-related products
AssigneeWacker Chemie AG
InventorRichard P. Burlingame
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Abstract
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.
Claims11
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) from E. Coli with reduced sensitivity to inhibition by serine in comparison to wild-type PGD;
- said DNA encoding PGD from E. Coli in which from 42 to 52 C-terminal amino acids have been deleted from the wild-type PGD of (SEQ ID NO:1) and in which an amino acid sequence has been inserted in place of the deleted C-terminal amino acids.
Dependencies are inferred from the claim text.